The invention relates to a preparing method of ephedra amination waste biosorbent. The preparing method includes the steps of 1, washing ephedra waste with tap water, washing the ephedra waste with distilled water, performing drying and crushing, and performing screening with a 40-mesh screen; 2, weighing the ephedra waste, and adding sodium hydroxide solution and epoxy chloropropane; 3, performing suction filtration, performing washing with the distilled water until neutralization, performing acetone washing, and performing drying to obtain ephedra waste epoxy cellulose ether for use; 4, adding the ephedra waste epoxy cellulose ether, diethylenetriamine, water and Na2CO3 into a container; allowing reaction at 20 DEG C to 120 DEG C for 0.5h to 2.5h; 5, performing suction filtration, performing washing with the distilled water until neutralization, performing ethanol rinsing, and performing drying to obtain the ephedra amination waste biosorbent. The ephedra amination waste biosorbent for heavy metal ions and dyes is prepared by chemical modification, and a novel way to recycle and deeply utilize the ephedra waste is provided.