The invention provides a shock isolation layer containing a mixture formed by glass bead mortar, glass beads and fine sand and a construction method thereof, belongs to the field of building structure foundation shock isolation and particularly relates to a shock isolation layer containing the mixture formed by the glass bead doped mortar, the glass beads and the fine sand and manufacture, wherein the shock isolation layer is laid between a foundation and an upper-portion structure. A plain concrete cushion layer is laid on the foundation. Steel sleeves and limiting steel bars are pre-embedded in a lower foundation beam, and the limiting steel bars penetrate the steel sleeves to reach to the top face of an upper foundation beam and are anchored through steel base plates and nuts. The shock isolation layer with the glass beads, the fine sand and mortar is laid between the upper foundation beam and the lower foundation beam. The steel sleeves are full of the fine sand. The shock isolation layer and the construction method are applicable to the foundation shock isolation of rural architectures and have very important significance on rural earthquake resistance and hazard mitigation. The construction method is simple, convenient, economic and effective and facilitates popularization and application of a large number of villages and small towns.