The invention discloses a surface acoustic micro-fluidic chip for tumor cell separation. The chip comprises a substrate, and a cover plate adhered on the substrate and provided with a micro-channel, wherein the micro-channel comprises a middle channel with an inlet and an outlet; cell sap injection channels connected with the inlet are arranged on two sides of the inlet; cell collection channels connected with the outlet are arranged on two sides of the outlet; two pairs of interdigital converters with identical frequencies and sound intensities are sequentially deposited on the substrate on two sides of the middle channel between the injection channel and the collection channel; the distance from the second pair of the interdigital converters to the middle channel is a quarter of surface wave length larger than that from the first pair of the interdigital converters to the middle channel; and small cell collection channels are symmetrically arranged on two sides of the middle channel between the two pairs of the interdigital converters. The chip has the advantages that a standing wave surface sound wave technology is realized, efficient, economic and pollution-free separation of tumor cell (CTCs) is realized, simultaneously the chip is low in cost, and the preparation technology is mature.