The invention relates to an ink capable of being cured on an automobile tire and a preparation method thereof. The ink comprises the following components in parts by weight: 6-25 parts of rubber, 40-70 parts of acrylate monomer, 5-25 parts of pigment pastes, 0.3-5 parts of flatting agent and 1-10 parts of dispersing agent. The ink curing mode is to carry out electron beam irradiation at room temperature. The ink has the beneficial effects that (1) the ink has proper applicable viscosity; (2) the adhesive force is 0 level; (3) a water resistance test can be up to 2300 hours; (4) an acid resistance test, an ink film does not abnormally change after being soaked by a 1% sulfuric acid solution for 24 hours; (5) an alkali resistance test, the ink film does not abnormally change after being soaked by a 1% sodium hydroxide solution for 24 hours; (6) the ink is high in efficiency, the electronic beam radiation dose is 1-500 KGY; and (7) no volatile organic compound (VOC) is discharged.