The invention belongs to the technology of cancer treatment, and in particular relates to preparation and application of an anti-cancer medicament carrier with dual functions of targeting and fluorescence. The preparation method for the carrier comprises the steps of: preparing mixed acid solution of concentrated sulfuric acid and concentrated nitric acid; preparing a short carbon nano tube; preparing a carbon nano tube-polyethylenimine aqueous-phase system; and preparing a difunctional carbon nano tube medicament carrier with targeting and fluorescence. The application method comprises the steps of: preparing an anti-cancer medicament paclitaxel-functionalized carbon nano tube composite; culturing cervical carcinoma cells and human umbilical vein endothelial cells; detecting the targeting of the multifunctional medicament carrier; and measuring the apoptosis rate. The preparation method and the application method have the advantages that: the carbon nano tube has high specific surface area, and the loading capacity is improved; quantum dots are narrow in emission spectrum, high in quantum yield and excellent in stability; and folic acid molecules improve the targeting of the medicament carrier. The functionalized carbon nano tube medicament carrier improves the aggregation of medicaments at a target position greatly and improves the effect of treating cancer by the medicament.